TERT Kit ELISA

N° du produit ABIN5659110

Détail du produit TERT Kit ELISA

Antigène: TERT (Telomerase Reverse Transcriptase (TERT))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Telomerase Reverse Transcriptase (TERT). No significant cross-reactivity or interference between Telomerase Reverse Transcriptase (TERT) and analogues was observed.

Sensibilité: 13.7 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomerase Reverse Transcriptase (TERT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Telomerase Reverse Transcriptase (TERT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Telomerase Reverse Transcriptase (TERT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Telomerase Reverse Transcriptase (TERT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Telomerase Reverse Transcriptase (TERT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Telomerase Reverse Transcriptase (TERT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TERT (Telomerase Reverse Transcriptase (TERT))

Autre désignation: Telomerase Reverse Transcriptase (TERT Produits)

Synonymes: CMM9 Kit ELISA, DKCA2 Kit ELISA, DKCB4 Kit ELISA, EST2 Kit ELISA, PFBMFT1 Kit ELISA, TCS1 Kit ELISA, TP2 Kit ELISA, TRT Kit ELISA, hEST2 Kit ELISA, hTRT Kit ELISA, chTERT Kit ELISA, Tert Kit ELISA, ATTERT Kit ELISA, F5E19.190 Kit ELISA, F5E19_190 Kit ELISA, telomerase reverse transcriptase Kit ELISA, tert Kit ELISA, TR Kit ELISA, est2 Kit ELISA, tcs1 Kit ELISA, telomerase Kit ELISA, tert-A Kit ELISA, tp2 Kit ELISA, trt Kit ELISA, xTERT Kit ELISA, telomerase reverse transcriptase Kit ELISA, Telomerase Reverse Transcriptase Kit ELISA, Tertp Kit ELISA, Telomerase catalytic subunit Kit ELISA, telomerase reverse transcriptase L homeolog Kit ELISA, TERT Kit ELISA, trt-1 Kit ELISA, Tert Kit ELISA, tert Kit ELISA, CMU_003490 Kit ELISA, VDBG_04487 Kit ELISA, GL50803_16225 Kit ELISA, tert.L Kit ELISA

Sujet:

Gene Name: Telomerase Reverse Transcriptase

Gene Aliases: EST2, TCS1, TP2, TRT, hEST2, Telomerase catalytic subunit, Telomerase-associated protein 2

ID gène: 301965

UniProt: Q673L6

Pathways: Telomere Maintenance