Thrombomodulin Kit ELISA

N° du produit ABIN5659166

Détail du produit Thrombomodulin Kit ELISA

Antigène: Thrombomodulin (THBD)

Reactivité: Chien

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Plasma

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Thrombomodulin (TM). No significant cross-reactivity or interference between Thrombomodulin (TM) and analogues was observed.

Sensibilité: 5.8 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombomodulin (TM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thrombomodulin (TM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombomodulin (TM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thrombomodulin (TM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombomodulin (TM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombomodulin (TM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Thrombomodulin (THBD)

Autre désignation: Thrombomodulin (THBD Produits)

Synonymes: AHUS6 Kit ELISA, BDCA3 Kit ELISA, CD141 Kit ELISA, THPH12 Kit ELISA, THRM Kit ELISA, TM Kit ELISA, AI385582 Kit ELISA, THBD Kit ELISA, HAST Kit ELISA, HAST3 Kit ELISA, M-PST Kit ELISA, ST1A3/ST1A4 Kit ELISA, ST1A5 Kit ELISA, STM Kit ELISA, TL-PST Kit ELISA, thrombomodulin Kit ELISA, sulfotransferase family 1A member 3 Kit ELISA, THBD Kit ELISA, Thbd Kit ELISA, SULT1A3 Kit ELISA

Sujet:

Gene Name: Thrombomodulin

Gene Aliases: CD141, THBD, BDCA3, THRM, Fetomodulin

ID gène: 474355

UniProt: Q5W7P8