TK1 Kit ELISA

N° du produit ABIN5659208

Détail du produit TK1 Kit ELISA

Antigène: TK1 (Thymidine Kinase 1, Soluble (TK1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Thymidine Kinase 1, Soluble (TK1). No significant cross-reactivity or interference between Thymidine Kinase 1, Soluble (TK1) and analogues was observed.

Sensibilité: 6.3 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidine Kinase 1, Soluble (TK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymidine Kinase 1, Soluble (TK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymidine Kinase 1, Soluble (TK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymidine Kinase 1, Soluble (TK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymidine Kinase 1, Soluble (TK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymidine Kinase 1, Soluble (TK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TK1 (Thymidine Kinase 1, Soluble (TK1))

Autre désignation: Thymidine Kinase 1, Soluble (TK1 Produits)

Synonymes: fi19b06 Kit ELISA, zgc:55362 Kit ELISA, wu:fi19b06 Kit ELISA, TK1 Kit ELISA, tk2 Kit ELISA, D530002A18Rik Kit ELISA, Tk-1 Kit ELISA, Tk1a Kit ELISA, Tk1b Kit ELISA, TK2 Kit ELISA, Tk Kit ELISA, TK Kit ELISA, thymidine kinase 1, soluble Kit ELISA, thymidine kinase 1 Kit ELISA, thymidine kinase, cytosolic Kit ELISA, Thymidine kinase, cytosolic Kit ELISA, temporal expression: early Kit ELISA, thymidine kinase Kit ELISA, Tk thymidine kinase Kit ELISA, tk1 Kit ELISA, TK1 Kit ELISA, EDI_049980 Kit ELISA, kith Kit ELISA, J2R Kit ELISA, HVT031 Kit ELISA, Tk1 Kit ELISA, tk Kit ELISA

Sujet:

Gene Name: Thymidine Kinase 1, Soluble

Gene Aliases: TK2, Thymidine kinase, cytosolic

ID gène: 7083

UniProt: P04183