TYMS Kit ELISA

N° du produit ABIN5659212

Détail du produit TYMS Kit ELISA

Antigène: TYMS (Thymidylate Synthetase (TYMS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Thymidylate Synthetase (TYMS). No significant cross-reactivity or interference between Thymidylate Synthetase (TYMS) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidylate Synthetase (TYMS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymidylate Synthetase (TYMS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymidylate Synthetase (TYMS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymidylate Synthetase (TYMS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymidylate Synthetase (TYMS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymidylate Synthetase (TYMS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TYMS (Thymidylate Synthetase (TYMS))

Autre désignation: Thymidylate Synthetase (TYMS Produits)

Synonymes: FN1 Kit ELISA, ECK2823 Kit ELISA, JW2795 Kit ELISA, ts Kit ELISA, Ts Kit ELISA, HST422 Kit ELISA, TMS Kit ELISA, TS Kit ELISA, thymidylate synthetase Kit ELISA, contains intron in T4 phage Kit ELISA, ThyE Kit ELISA, thymidylate synthase Kit ELISA, thymidylate synthetase L homeolog Kit ELISA, TYMS Kit ELISA, thyA Kit ELISA, td Kit ELISA, thyE Kit ELISA, DDA3937_RS04960 Kit ELISA, tyms.L Kit ELISA, XBJ1_RS15770 Kit ELISA, EAMY_RS20680 Kit ELISA, Tyms Kit ELISA

Classe de substances: Viral Protein

Sujet:

Gene Name: Thymidylate Synthetase

Gene Aliases: TS, TMS, TSase, Thymidylate Synthase

ID gène: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases