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TMSB4Y Kit ELISA

TMSB4Y Reactivité: Humain Colorimetric Competition ELISA 123.46 ng/mL - 10000 ng/mL Plasma, Serum
N° du produit ABIN5659217
  • Antigène Voir toutes TMSB4Y Kits ELISA
    TMSB4Y (Thymosin beta 4, Y-Linked (TMSB4Y))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    123.46 ng/mL - 10000 ng/mL
    Seuil minimal de détection
    123.46 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Thymosin Beta 4, Y-Chromosomal (Tb4Y). No significant cross-reactivity or interference between Thymosin Beta 4, Y-Chromosomal (Tb4Y) and analogues was observed.
    Sensibilité
    42.2 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Thymosin Beta 4, Y-Chromosomal (Tb4Y) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Thymosin Beta 4, Y-Chromosomal (Tb4Y) and unlabeled Thymosin Beta 4, Y-Chromosomal (Tb4Y) (Standards or samples) with the pre-coated antibody specific to Thymosin Beta 4, Y-Chromosomal (Tb4Y). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Thymosin Beta 4, Y-Chromosomal (Tb4Y) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Thymosin Beta 4, Y-Chromosomal (Tb4Y) in the sample.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymosin Beta 4, Y-Chromosomal (Tb4Y) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymosin Beta 4, Y-Chromosomal (Tb4Y) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes TMSB4Y Kits ELISA
    TMSB4Y (Thymosin beta 4, Y-Linked (TMSB4Y))
    Autre désignation
    Thymosin Beta 4, Y-Chromosomal (TMSB4Y Produits)
    Synonymes
    TB4Y Kit ELISA, thymosin beta 4, Y-linked Kit ELISA, TMSB4Y Kit ELISA
    Sujet

    Gene Name: Thymosin Beta 4, Y-Chromosomal

    Gene Aliases: TMSB4Y, Thymosin,Beta 4,Y-Linked

    Pathways
    Regulation of Actin Filament Polymerization, Maintenance of Protein Location
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