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TFAM Kit ELISA

TFAM Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5659393
  • Antigène Voir toutes TFAM Kits ELISA
    TFAM (Transcription Factor A, Mitochondrial (TFAM))
    Reactivité
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Transcription Factor A, Mitochondrial (TFAM). No significant cross-reactivity or interference between Transcription Factor A, Mitochondrial (TFAM) and analogues was observed.
    Sensibilité
    0.124 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor A, Mitochondrial (TFAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor A, Mitochondrial (TFAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor A, Mitochondrial (TFAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transcription Factor A, Mitochondrial (TFAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transcription Factor A, Mitochondrial (TFAM) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transcription Factor A, Mitochondrial (TFAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes TFAM Kits ELISA
    TFAM (Transcription Factor A, Mitochondrial (TFAM))
    Autre désignation
    Transcription Factor A, Mitochondrial (TFAM Produits)
    Synonymes
    tcf6 Kit ELISA, mttf1 Kit ELISA, mttfa Kit ELISA, tcf6l1 Kit ELISA, tcf6l2 Kit ELISA, tcf6l3 Kit ELISA, mttfa-A Kit ELISA, xl-mtTFA Kit ELISA, MGC153358 Kit ELISA, zgc:153358 Kit ELISA, MTTF1 Kit ELISA, MTTFA Kit ELISA, TCF6 Kit ELISA, TCF6L1 Kit ELISA, TCF6L2 Kit ELISA, TCF6L3 Kit ELISA, AI661103 Kit ELISA, Hmgts Kit ELISA, mtTFA Kit ELISA, tsHMG Kit ELISA, Mttfa Kit ELISA, transcription factor A, mitochondrial Kit ELISA, transcription factor A, mitochondrial L homeolog Kit ELISA, TFAM Kit ELISA, tfam.L Kit ELISA, tfam Kit ELISA, Tfam Kit ELISA
    Sujet

    Gene Name: Transcription Factor A, Mitochondrial

    Gene Aliases: MtTF1, TCF6, TCF6L2, mtTFA, Mitochondrial transcription factor 1, Transcription factor 6-like 2

    ID gène
    7019
    UniProt
    Q00059
    Pathways
    Chromatin Binding
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