Transglutaminase 2 Kit CLIA

N° du produit ABIN5659474

Détail du produit Transglutaminase 2 Kit CLIA

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 2.5 ng/mL - 160 ng/mL

Seuil minimal de détection: 2.5 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Transglutaminase 2, Tissue (TGM2). No significant cross-reactivity or interference between Transglutaminase 2, Tissue (TGM2) and analogues was observed.

Sensibilité: 0.11 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Transglutaminase 2, Tissue (TGM2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transglutaminase 2, Tissue (TGM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transglutaminase 2, Tissue (TGM2) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transglutaminase 2, Tissue (TGM2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transglutaminase 2, Tissue (TGM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Autre désignation: Transglutaminase 2, Tissue (TGM2 Produits)

Synonymes: G-ALPHA-h Kit CLIA, GNAH Kit CLIA, TG2 Kit CLIA, TGC Kit CLIA, tgm2 Kit CLIA, zgc:101081 Kit CLIA, TgaseII Kit CLIA, TGM2 Kit CLIA, fc12b04 Kit CLIA, wu:fc12b04 Kit CLIA, zgc:136700 Kit CLIA, TGL2 Kit CLIA, G[a]h Kit CLIA, TGase2 Kit CLIA, tTG Kit CLIA, tTGas Kit CLIA, transglutaminase 2 Kit CLIA, transglutaminase 2, C polypeptide A Kit CLIA, transglutaminase 2b Kit CLIA, transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) Kit CLIA, transglutaminase 2, C polypeptide Kit CLIA, TGM2 Kit CLIA, tgm2a Kit CLIA, Tgm2 Kit CLIA, tgm2b Kit CLIA

Sujet:

Gene Name: Transglutaminase 2, Tissue

Gene Aliases: tTG, TG2, C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase, Transglutaminase C, Transglutaminase H

ID gène: 7052

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling