TTR Kit ELISA

N° du produit ABIN5659513

Détail du produit TTR Kit ELISA

Antigène: TTR (Transthyretin (TTR))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Transthyretin (TTR). No significant cross-reactivity or interference between Transthyretin (TTR) and analogues was observed.

Sensibilité: 0.071 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transthyretin (TTR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transthyretin (TTR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transthyretin (TTR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transthyretin (TTR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transthyretin (TTR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transthyretin (TTR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TTR (Transthyretin (TTR))

Autre désignation: Transthyretin (TTR Produits)

Synonymes: zgc:103583 Kit ELISA, TTR Kit ELISA, LOC100226992 Kit ELISA, CTS Kit ELISA, CTS1 Kit ELISA, HsT2651 Kit ELISA, PALB Kit ELISA, TBPA Kit ELISA, AA408768 Kit ELISA, AI787086 Kit ELISA, D17860 Kit ELISA, prealbumin Kit ELISA, Lr1 Kit ELISA, Tbpa Kit ELISA, Prealbumin Kit ELISA, xTTR Kit ELISA, transthyretin (prealbumin, amyloidosis type I) Kit ELISA, transthyretin Kit ELISA, Transthyretin Kit ELISA, transthyretin (prealbumin, amyloidosis type 1) Kit ELISA, transthyretin-like Kit ELISA, transthyretin L homeolog Kit ELISA, ttr Kit ELISA, TTR Kit ELISA, vpb38 Kit ELISA, MRAD2831_RS38185 Kit ELISA, Caci_4246 Kit ELISA, LOC100226992 Kit ELISA, Ttr Kit ELISA, ttr.L Kit ELISA

Sujet:

Gene Name: Transthyretin

Gene Aliases: TBPA, PA, PLB, ATTR, PALB, Prealbumin, Prealbumin Amyloidosis Type I, Transports Thyroxine and Retinol

ID gène: 22139

UniProt: P07309

Pathways: Hormone Transport