TUBB Kit ELISA

N° du produit ABIN5659603

Détail du produit TUBB Kit ELISA

Antigène: TUBB (Tubulin, beta (TUBB))

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tubulin Beta (TUBb). No significant cross-reactivity or interference between Tubulin Beta (TUBb) and analogues was observed.

Sensibilité: 12.7 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tubulin Beta (TUBb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tubulin Beta (TUBb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tubulin Beta (TUBb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tubulin Beta (TUBb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tubulin Beta (TUBb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tubulin Beta (TUBb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TUBB (Tubulin, beta (TUBB))

Autre désignation: Tubulin Beta (TUBB Produits)

Synonymes: M40 Kit ELISA, OK/SW-cl.56 Kit ELISA, TUBB1 Kit ELISA, TUBB5 Kit ELISA, XLOT Kit ELISA, m40 Kit ELISA, ok/sw-cl.56 Kit ELISA, tubb1 Kit ELISA, tubb5 Kit ELISA, TUBB2A Kit ELISA, TUBB2B Kit ELISA, TUBB Kit ELISA, 1t Kit ELISA, B1t Kit ELISA, BETA 56D Kit ELISA, CG9277 Kit ELISA, DTB2 Kit ELISA, Dmbeta1 Kit ELISA, Dmel\\CG9277 Kit ELISA, T Kit ELISA, Tub Kit ELISA, Tubulin Kit ELISA, beta Kit ELISA, beta-Tub Kit ELISA, beta-Tub56D Kit ELISA, beta-tub Kit ELISA, beta-tubulin56D Kit ELISA, beta1 Kit ELISA, beta1-Tubulin Kit ELISA, beta1-tub Kit ELISA, beta1Tub Kit ELISA, beta1t Kit ELISA, beta1tub Kit ELISA, beta56D Kit ELISA, betaTub Kit ELISA, betaTub1 Kit ELISA, beta[[1]] tubulin Kit ELISA, beta[[1]]-tubulin Kit ELISA, betatub(56D) Kit ELISA, 143391_i_at Kit ELISA, 3t Kit ELISA, B3t Kit ELISA, BETA 60D Kit ELISA, CG3401 Kit ELISA, D.m.BETA-60D Kit ELISA, DTB3 Kit ELISA, Dmbeta3 Kit ELISA, Dmel\\CG3401 Kit ELISA, Tub60D Kit ELISA, beta-Tub60D Kit ELISA, beta-Tub6D Kit ELISA, beta3 Kit ELISA, beta3 TU Kit ELISA, beta3-Tub Kit ELISA, beta3-tubulin Kit ELISA, beta3TUB Kit ELISA, beta3Tub Kit ELISA, beta3t Kit ELISA, beta60C Kit ELISA, betaTub3 Kit ELISA, betaTub60C Kit ELISA, beta[[3]] tubulin Kit ELISA, beta[[3]]-Tub Kit ELISA, beta[[3]]-tubulin Kit ELISA, betatub60D Kit ELISA, p50 Kit ELISA, p50/tubulin Kit ELISA, p53 Kit ELISA, p53/tubulin Kit ELISA, 2t Kit ELISA, B2t Kit ELISA, BETA 85D Kit ELISA, BETA2 Kit ELISA, CG9359 Kit ELISA, D.m.BETA-85D Kit ELISA, DTB4 Kit ELISA, Dmbeta2 Kit ELISA, Dmel\\CG9359 Kit ELISA, beta(2)Tu Kit ELISA, beta(2)Tub Kit ELISA, beta-Tub85D Kit ELISA, beta-tub85D Kit ELISA, beta2 Kit ELISA, beta2-tub Kit ELISA, beta2t Kit ELISA, beta2tub Kit ELISA, beta85D Kit ELISA, betaTub2 Kit ELISA, beta[[2]]-tubulin Kit ELISA, ms(3)KK[D] Kit ELISA, 4t Kit ELISA, B4t Kit ELISA, BETA 98B Kit ELISA, CG4869 Kit ELISA, DTB1 Kit ELISA, Dmbeta4 Kit ELISA, Dmel\\CG4869 Kit ELISA, beta-Tub97EF Kit ELISA, beta4 Kit ELISA, beta4t Kit ELISA, beta97F Kit ELISA, betaTub4 Kit ELISA, betaTub98BC Kit ELISA, betaTub98C Kit ELISA, Tub1 Kit ELISA, bmtub1 Kit ELISA, Tub3 Kit ELISA, bmtub3 Kit ELISA, Tub2 Kit ELISA, bmtub2 Kit ELISA, Tub4 Kit ELISA, bmtub4 Kit ELISA, LOC100101153 Kit ELISA, tubulin beta class I Kit ELISA, putative cobalt-zinc-cadmium outer membrane resistance protein Kit ELISA, Tubulin beta chain Kit ELISA, tubulin beta-2A chain Kit ELISA, beta-Tubulin at 56D Kit ELISA, beta-Tubulin at 60D Kit ELISA, beta-Tubulin at 85D Kit ELISA, beta-Tubulin at 97EF Kit ELISA, beta-tubulin Kit ELISA, tubulin beta 6 class V Kit ELISA, beta tubulin Kit ELISA, tubulin beta class I L homeolog Kit ELISA, tubulin, beta 5 class I Kit ELISA, TUBB Kit ELISA, czcC Kit ELISA, tbb-6 Kit ELISA, tubb Kit ELISA, LOC462399 Kit ELISA, betaTub56D Kit ELISA, betaTub60D Kit ELISA, betaTub85D Kit ELISA, betaTub97EF Kit ELISA, Tub1 Kit ELISA, Tub3 Kit ELISA, Tub2 Kit ELISA, Tub4 Kit ELISA, TVAG_525430 Kit ELISA, TVAG_062880 Kit ELISA, TVAG_456920 Kit ELISA, LOC100101153 Kit ELISA, TUBB6 Kit ELISA, Tb927.1.2330 Kit ELISA, Tb927.1.2350 Kit ELISA, Tb927.1.2370 Kit ELISA, Tb927.1.2390 Kit ELISA, tubb.L Kit ELISA, Tubb Kit ELISA, LOC693049 Kit ELISA, LOC443015 Kit ELISA, Tubb5 Kit ELISA

Sujet:

Gene Name: Tubulin Beta

Gene Aliases: TUB-B, TUBB5, TUBB1, M40

Pathways: Dynamique des Microtubules, M Phase