Tyrosine Hydroxylase Kit ELISA

N° du produit ABIN5659712

Détail du produit Tyrosine Hydroxylase Kit ELISA

Antigène: Tyrosine Hydroxylase (TH)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tyrosine Hydroxylase (TH). No significant cross-reactivity or interference between Tyrosine Hydroxylase (TH) and analogues was observed.

Sensibilité: 0.062 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine Hydroxylase (TH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosine Hydroxylase (TH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine Hydroxylase (TH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosine Hydroxylase (TH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosine Hydroxylase (TH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosine Hydroxylase (TH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Tyrosine Hydroxylase (TH)

Autre désignation: Tyrosine Hydroxylase (TH Produits)

Synonymes: DYT14 Kit ELISA, DYT5b Kit ELISA, TYH Kit ELISA, The Kit ELISA, CG10118 Kit ELISA, DH65B Kit ELISA, DTH Kit ELISA, Dmel\\CG10118 Kit ELISA, Pale Kit ELISA, Ple Kit ELISA, TH Kit ELISA, Th Kit ELISA, VII Kit ELISA, dTH1 Kit ELISA, dTH65B Kit ELISA, pale/ple Kit ELISA, th Kit ELISA, dyt14 Kit ELISA, dyt5b Kit ELISA, tyh Kit ELISA, tyrosine hydroxylase Kit ELISA, pale Kit ELISA, tyrosine 3-monooxygenase Kit ELISA, tyrosine hydroxylase S homeolog Kit ELISA, TH Kit ELISA, Th Kit ELISA, ple Kit ELISA, th Kit ELISA, th.S Kit ELISA, LOC100008895 Kit ELISA

Sujet:

Gene Name: Tyrosine Hydroxylase

Gene Aliases: TYH, DYT5b, Tyrosine 3-Monooxygenase, Dystonia 14

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour