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URGCP Kit ELISA

URGCP Reactivité: Souris Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5659761
  • Antigène Voir toutes URGCP Kits ELISA
    URGCP (Upregulator of Cell Proliferation (URGCP))
    Reactivité
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Upregulator Of Cell Proliferation (URGCP). No significant cross-reactivity or interference between Upregulator Of Cell Proliferation (URGCP) and analogues was observed.
    Sensibilité
    0.061 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Upregulator Of Cell Proliferation (URGCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Upregulator Of Cell Proliferation (URGCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Upregulator Of Cell Proliferation (URGCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Upregulator Of Cell Proliferation (URGCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Upregulator Of Cell Proliferation (URGCP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Upregulator Of Cell Proliferation (URGCP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes URGCP Kits ELISA
    URGCP (Upregulator of Cell Proliferation (URGCP))
    Autre désignation
    Upregulator Of Cell Proliferation (URGCP Produits)
    Synonymes
    URG4 Kit ELISA, DKFZp469D1721 Kit ELISA, urg4 Kit ELISA, MGC132266 Kit ELISA, 2010005J08Rik Kit ELISA, 9130001I21Rik Kit ELISA, AW060359 Kit ELISA, Urg4 Kit ELISA, mKIAA1507 Kit ELISA, RGD1564681 Kit ELISA, up-regulator of cell proliferation Kit ELISA, upregulator of cell proliferation Kit ELISA, LOC610578 Kit ELISA, LOC100064842 Kit ELISA, URGCP Kit ELISA, urgcp Kit ELISA, Urgcp Kit ELISA
    Sujet

    Gene Name: Upregulator Of Cell Proliferation

    Gene Aliases: URG4, Up Regulated Gene 4, HBV X protein up-regulated gene 4 protein, HBxAg up-regulated gene 4 protein

    ID gène
    72046
    UniProt
    Q5NCI0
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