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Versican Kit ELISA

Vcan Reactivité: Souris Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5659920
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    Versican (Vcan)
    Reactivité
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    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Versican (VS). No significant cross-reactivity or interference between Versican (VS) and analogues was observed.
    Sensibilité
    0.066 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Versican (VS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Versican (VS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Versican (VS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Versican (VS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Versican (VS) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Versican (VS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Versican (Vcan) Kits ELISA
    Versican (Vcan)
    Autre désignation
    Versican (Vcan Produits)
    Synonymes
    br146 Kit ELISA, cspg2 Kit ELISA, cspg2a Kit ELISA, sb:cb63 Kit ELISA, versican Kit ELISA, CSPG2 Kit ELISA, Cspg2 Kit ELISA, wgn Kit ELISA, ervr Kit ELISA, pg-m Kit ELISA, wgn1 Kit ELISA, Xversican Kit ELISA, dcan Kit ELISA, cspg2b Kit ELISA, fb99b07 Kit ELISA, wu:fb99b07 Kit ELISA, ERVR Kit ELISA, GHAP Kit ELISA, PG-M Kit ELISA, WGN Kit ELISA, WGN1 Kit ELISA, 5430420N07Rik Kit ELISA, 9430051N09 Kit ELISA, DPEAAE Kit ELISA, NG2 Kit ELISA, PG-M(V0) Kit ELISA, PG-M(V1) Kit ELISA, hdf Kit ELISA, versican a Kit ELISA, versican Kit ELISA, versican b Kit ELISA, vcana Kit ELISA, VCAN Kit ELISA, Vcan Kit ELISA, vcan Kit ELISA, vcanb Kit ELISA
    Sujet

    Gene Name: Versican

    Gene Aliases: CSPG2, VCAN, ERVR, PG-M, WGN1, Chondroitin Sulfate Proteoglycan 2, Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding, Large fibroblast proteoglycan

    UniProt
    Q62059
    Pathways
    Glycosaminoglycan Metabolic Process
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