Vinculin Kit ELISA

N° du produit ABIN5659937

Détail du produit Vinculin Kit ELISA

Antigène: Vinculin (VCL)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Vinculin (VCL). No significant cross-reactivity or interference between Vinculin (VCL) and analogues was observed.

Sensibilité: 0.128 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vinculin (VCL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vinculin (VCL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vinculin (VCL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vinculin (VCL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vinculin (VCL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vinculin (VCL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Vinculin (VCL)

Autre désignation: Vinculin (VCL Produits)

Synonymes: CMD1W Kit ELISA, CMH15 Kit ELISA, MV Kit ELISA, MVCL Kit ELISA, VCL Kit ELISA, im:6902790 Kit ELISA, hm:zehn2160 Kit ELISA, DDBDRAFT_0186762 Kit ELISA, DDBDRAFT_0232320 Kit ELISA, DDB_0186762 Kit ELISA, DDB_0232320 Kit ELISA, 9430097D22 Kit ELISA, AA571387 Kit ELISA, AI462105 Kit ELISA, AW545629 Kit ELISA, CG3299 Kit ELISA, CT11081 Kit ELISA, Dmel\\CG3299 Kit ELISA, Dmvincp Kit ELISA, EG:103B4.1 Kit ELISA, Vin Kit ELISA, Vin2EF Kit ELISA, vinc Kit ELISA, LOC100228062 Kit ELISA, vcl-A Kit ELISA, vinculin Kit ELISA, vinculin a Kit ELISA, vinculin A Kit ELISA, Vinculin Kit ELISA, vinculin S homeolog Kit ELISA, VCL Kit ELISA, vcla Kit ELISA, vinA Kit ELISA, Vcl Kit ELISA, Vinc Kit ELISA, CpipJ_CPIJ013873 Kit ELISA, Bm1_37935 Kit ELISA, vcl Kit ELISA, vcl.S Kit ELISA

Sujet:

Gene Name: Vinculin

Gene Aliases: MVCL, Metavinculin

ID gène: 22330

UniProt: Q64727

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2