NAMPT Kit ELISA

N° du produit ABIN5659946

Détail du produit NAMPT Kit ELISA

Antigène: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Visfatin (VF). No significant cross-reactivity or interference between Visfatin (VF) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Visfatin (VF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Visfatin (VF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Visfatin (VF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Visfatin (VF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Visfatin (VF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Visfatin (VF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Autre désignation: Visfatin (NAMPT Produits)

Synonymes: 1110035O14Rik Kit ELISA, PBEF Kit ELISA, PBEF1 Kit ELISA, VF Kit ELISA, VISFATIN Kit ELISA, AI314458 Kit ELISA, AI480535 Kit ELISA, NAmPRTase Kit ELISA, Pbef Kit ELISA, Pbef1 Kit ELISA, Visfatin Kit ELISA, visfatin Kit ELISA, pbef Kit ELISA, pbef1 Kit ELISA, nicotinamide phosphoribosyltransferase Kit ELISA, Nicotinamide phosphoribosyltransferase Kit ELISA, nicotinamide phosphoribosyltransferase S homeolog Kit ELISA, NAMPT Kit ELISA, Nampt Kit ELISA, nampt Kit ELISA, Smon_0680 Kit ELISA, Mrub_2845 Kit ELISA, Cseg_3704 Kit ELISA, BC1002_3997 Kit ELISA, Ftrac_2686 Kit ELISA, Varpa_5681 Kit ELISA, Celal_3900 Kit ELISA, Deima_0880 Kit ELISA, BC1001_3724 Kit ELISA, Celly_1696 Kit ELISA, Clole_1542 Kit ELISA, Tsp_05934 Kit ELISA, nampt.S Kit ELISA

Sujet:

Gene Name: Visfatin

Gene Aliases: Nampt, NAmPRTase, PBEF, PBEF1, Pre-B-Cell Colony Enhancing Factor, Nicotinamide Phosphoribosyltransferase

ID gène: 10135

UniProt: P43490