Vitamin D-Binding Protein Kit CLIA

N° du produit ABIN5659955

Détail du produit Vitamin D-Binding Protein Kit CLIA

Antigène: Vitamin D-Binding Protein (GC)

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 3.91 ng/mL - 1000 ng/mL

Seuil minimal de détection: 3.91 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Vitamin D Binding Protein (DBP). No significant cross-reactivity or interference between Vitamin D Binding Protein (DBP) and analogues was observed.

Sensibilité: 0.59 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein (DBP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Vitamin D Binding Protein (DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Vitamin D Binding Protein (DBP) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D Binding Protein (DBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Vitamin D-Binding Protein (GC)

Autre désignation: Vitamin D Binding Protein (GC Produits)

Synonymes: DBP Kit CLIA, DBP/GC Kit CLIA, GRD3 Kit CLIA, VDBG Kit CLIA, VDBP Kit CLIA, zgc:110389 Kit CLIA, zgc:92753 Kit CLIA, GC Kit CLIA, Gc Kit CLIA, DBP02 Kit CLIA, VDB Kit CLIA, Vdbp Kit CLIA, VTDB Kit CLIA, gc Kit CLIA, GC, vitamin D binding protein Kit CLIA, D-box binding PAR bZIP transcription factor Kit CLIA, D site albumin promoter binding protein Kit CLIA, group-specific component (vitamin D binding protein) Kit CLIA, group specific component Kit CLIA, group-specific component (vitamin D binding protein) S homeolog Kit CLIA, GC Kit CLIA, Dbp Kit CLIA, gc Kit CLIA, Gc Kit CLIA, gc.S Kit CLIA

Sujet:

Gene Name: Vitamin D Binding Protein

Gene Aliases: GC, VDBG, VDBP, Group-Specific Component, Gc-globulin

ID gène: 2638

UniProt: P02774

Pathways: Metabolism of Steroid Hormones and Vitamin D, Monocarboxylic Acid Catabolic Process