Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Vitamin D Receptor Kit ELISA

VDR Reactivité: Rat Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5659961
  • Antigène Voir toutes Vitamin D Receptor (VDR) Kits ELISA
    Vitamin D Receptor (VDR)
    Reactivité
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.625 ng/mL - 40 ng/mL
    Seuil minimal de détection
    0.625 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Vitamin D Receptor (VDR). No significant cross-reactivity or interference between Vitamin D Receptor (VDR) and analogues was observed.
    Sensibilité
    0.244 ng/mL
    Featured
    Discover our best selling VDR Kit ELISA
    Top Product
    Discover our top product VDR Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Receptor (VDR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Receptor (VDR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Receptor (VDR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D Receptor (VDR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D Receptor (VDR) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D Receptor (VDR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Vitamin D Receptor (VDR) Kits ELISA
    Vitamin D Receptor (VDR)
    Autre désignation
    Vitamin D Receptor (VDR Produits)
    Synonymes
    vdrbeta Kit ELISA, vdr0 Kit ELISA, LOC100136219 Kit ELISA, NR1I1-B Kit ELISA, gb:dq017633 Kit ELISA, vdr-b Kit ELISA, Ci-VDR-b Kit ELISA, VDR Kit ELISA, LOC100221284 Kit ELISA, vdr-A Kit ELISA, xVDR Kit ELISA, Nr1i1 Kit ELISA, vdr Kit ELISA, NR1I1 Kit ELISA, PPP1R163 Kit ELISA, vitamin D (1,25- dihydroxyvitamin D3) receptor Kit ELISA, vitamin D receptor Kit ELISA, vitamin D3 receptor A Kit ELISA, vitamin D receptor b Kit ELISA, nuclear receptor VDR-b Kit ELISA, vitamin D (1,25- dihydroxyvitamin D3) receptor L homeolog Kit ELISA, vitamin D (1,25-dihydroxyvitamin D3) receptor Kit ELISA, vitamin D receptor a Kit ELISA, vdr Kit ELISA, vdr0 Kit ELISA, VDR Kit ELISA, LOC100136219 Kit ELISA, vdrb Kit ELISA, vdr-b Kit ELISA, vdr.L Kit ELISA, Vdr Kit ELISA, vdra Kit ELISA
    Classe de substances
    Chemical
    Sujet

    Gene Name: Vitamin D Receptor

    Gene Aliases: NR1I1, Nuclear Receptor Subfamily 1 Group I Member 1, Calcitriol Receptor, 1,25-dihydroxyvitamin D3 receptor

    Pathways
    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
Vous êtes ici:
Support technique