WT1 Kit CLIA

N° du produit ABIN5659991

Kit ELISA Humain WT1, test Chemiluminescent pour la quantification de Humain WT1.

Aperçu rapide pour WT1 Kit CLIA (ABIN5659991)

Antigène: WT1 (Wilms Tumor 1 (WT1))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Détail du produit WT1 Kit CLIA

Seuil minimal de détection: 156.25 pg/mL

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Wilms Tumor Protein (WT1). No significant cross-reactivity or interference between Wilms Tumor Protein (WT1) and analogues was observed.

Sensibilité: 6.21 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Wilms Tumor Protein (WT1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wilms Tumor Protein (WT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wilms Tumor Protein (WT1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: WT1 (Wilms Tumor 1 (WT1))

Autre désignation: Wilms Tumor Protein

Sujet:

Gene Name: Wilms Tumor Protein

Gene Aliases: WAGR, GUD, WIT-2, WT33

ID gène: 7490

UniProt: P19544

Pathways: Tube Formation