WT1 Kit ELISA

N° du produit ABIN5659992

Détail du produit WT1 Kit ELISA

Antigène: WT1 (Wilms Tumor 1 (WT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Wilms Tumor Protein (WT1). No significant cross-reactivity or interference between Wilms Tumor Protein (WT1) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wilms Tumor Protein (WT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wilms Tumor Protein (WT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wilms Tumor Protein (WT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wilms Tumor Protein (WT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wilms Tumor Protein (WT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: WT1 (Wilms Tumor 1 (WT1))

Autre désignation: Wilms Tumor Protein (WT1 Produits)

Synonymes: AWT1 Kit ELISA, EWS-WT1 Kit ELISA, GUD Kit ELISA, NPHS4 Kit ELISA, WAGR Kit ELISA, WIT-2 Kit ELISA, WT33 Kit ELISA, CWT1 Kit ELISA, WT1 Kit ELISA, XWT1a Kit ELISA, wt-1 Kit ELISA, wt1 Kit ELISA, xWT1 Kit ELISA, WT-1 Kit ELISA, gud Kit ELISA, wagr Kit ELISA, wt33 Kit ELISA, xwt1 Kit ELISA, wit-2 Kit ELISA, si:ch211-160j6.3 Kit ELISA, zgc:152697 Kit ELISA, D630046I19Rik Kit ELISA, Wt-1 Kit ELISA, XWT1b Kit ELISA, XeWT1 Kit ELISA, Wilms tumor 1 Kit ELISA, Wilms tumor 1 S homeolog Kit ELISA, wilms tumor 1b Kit ELISA, Wilms tumor protein Kit ELISA, Wilms tumor 1 homolog Kit ELISA, Wilms tumor 1 L homeolog Kit ELISA, WT1 Kit ELISA, wt1.S Kit ELISA, wt1 Kit ELISA, wt1b Kit ELISA, Wt1 Kit ELISA, wt1.L Kit ELISA

Sujet:

Gene Name: Wilms Tumor Protein

Gene Aliases: WAGR, GUD, WIT-2, WT33

ID gène: 7490

UniProt: P19544

Pathways: Tube Formation