ATP1A1 Kit ELISA

N° du produit ABIN5674661

Détail du produit ATP1A1 Kit ELISA

Antigène: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 26.6 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Autre désignation: ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) (ATP1A1 Produits)

Synonymes: Atpa-1 Kit ELISA, BC010319 Kit ELISA, Nkaa1b Kit ELISA, K-ATPase Kit ELISA, Na+K+ATPase Kit ELISA, atp1a1 Kit ELISA, atp1a1a.1 Kit ELISA, atp1a1l1 Kit ELISA, atp[a]1B1 Kit ELISA, cb70 Kit ELISA, wu:fa03c03 Kit ELISA, wu:fb92h10 Kit ELISA, atp1a1l5 Kit ELISA, atp[a]1B2 Kit ELISA, cb707 Kit ELISA, wu:fj34g01 Kit ELISA, zgc:92351 Kit ELISA, ATPase Na+/K+ transporting subunit alpha 1 Kit ELISA, ATPase, Na+/K+ transporting, alpha 1 polypeptide Kit ELISA, ATPase Na+/K+ transporting subunit alpha 1 L homeolog Kit ELISA, ATPase, Na+/K+ transporting, alpha 1a polypeptide, tandem duplicate 1 Kit ELISA, ATPase, Na+/K+ transporting, alpha 1b polypeptide Kit ELISA, ATP1A1 Kit ELISA, Atp1a1 Kit ELISA, atp1a1.L Kit ELISA, atp1a1a.1 Kit ELISA, atp1a1b Kit ELISA

Sujet: Alternative name: Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium/potassium-transporting ATPase subunit alpha-1

ID gène: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process