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Apelin Receptor Kit ELISA

APLNR Reactivité: Souris Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Tissue Homogenate
N° du produit ABIN5674667
  • Antigène Voir toutes Apelin Receptor (APLNR) Kits ELISA
    Apelin Receptor (APLNR)
    Reactivité
    • 3
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.625 pg/mL - 1000 pg/mL
    Seuil minimal de détection
    15.625 pg/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    5.9 pg/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes Apelin Receptor (APLNR) Kits ELISA
    Apelin Receptor (APLNR)
    Abstract
    APLNR Produits
    Synonymes
    AGTRL1 Kit ELISA, APJ Kit ELISA, APJR Kit ELISA, HG11 Kit ELISA, Agtrl1 Kit ELISA, msr/apj Kit ELISA, Apj Kit ELISA, X-msr Kit ELISA, Xangio1 Kit ELISA, Xmsr Kit ELISA, agtrl1 Kit ELISA, angio1 Kit ELISA, apj Kit ELISA, apjr Kit ELISA, hg11 Kit ELISA, msr Kit ELISA, APLNR Kit ELISA, DKFZp469C1532 Kit ELISA, agtrl1-B Kit ELISA, agtrl1b Kit ELISA, Agtrl1a Kit ELISA, grn Kit ELISA, zgc:114063 Kit ELISA, APJa Kit ELISA, XAngio1 Kit ELISA, agtrl1-A Kit ELISA, agtrl1a Kit ELISA, aplnr-a Kit ELISA, aplnr-b Kit ELISA, x-MSR Kit ELISA, xAngio1 Kit ELISA, APLNR1 Kit ELISA, apelin receptor Kit ELISA, apelin receptor S homeolog Kit ELISA, apelin receptor b Kit ELISA, apelin receptor L homeolog Kit ELISA, APLNR Kit ELISA, Aplnr Kit ELISA, aplnr Kit ELISA, aplnr.S Kit ELISA, aplnrb Kit ELISA, aplnr.L Kit ELISA
    Sujet
    Alternative name: AGTRL1, HG11, APJR, APJ, APJ Receptor, Angiotensin II Receptor-Like 1, Angiotensin receptor-like 1
    ID gène
    23796
    UniProt
    Q9WV08
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