Carboxypeptidase A2 Kit ELISA

N° du produit ABIN5674679

Détail du produit Carboxypeptidase A2 Kit ELISA

Antigène: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Sensibilité: 0047 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Autre désignation: Carboxypeptidase A2, Pancreatic (CPA2) (CPA2 Produits)

Synonymes: CPA2 Kit ELISA, zgc:92530 Kit ELISA, carboxypeptidase A2 Kit ELISA, carboxypeptidase A2 (pancreatic) Kit ELISA, Carboxypeptidase A2 Kit ELISA, carboxypeptidase A2, pancreatic Kit ELISA, CPA2 Kit ELISA, cpa2 Kit ELISA, CpipJ_CPIJ001743 Kit ELISA, cbpa2 Kit ELISA, Cpa2 Kit ELISA

ID gène: 1358

UniProt: P48052