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Endothelin 3 Kit ELISA

EDN3 Reactivité: Souris Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5674683
  • Antigène Voir toutes Endothelin 3 (EDN3) Kits ELISA
    Endothelin 3 (EDN3)
    Reactivité
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    14.9 pg/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes Endothelin 3 (EDN3) Kits ELISA
    Endothelin 3 (EDN3)
    Abstract
    EDN3 Produits
    Synonymes
    et-3 Kit ELISA, EDN3 Kit ELISA, ET-3 Kit ELISA, ET3 Kit ELISA, HSCR4 Kit ELISA, PPET3 Kit ELISA, WS4B Kit ELISA, 114CH19 Kit ELISA, ls Kit ELISA, tmgc48 Kit ELISA, Et3 Kit ELISA, RGD1564825 Kit ELISA, preproET-3 Kit ELISA, endothelin-3 L homeolog Kit ELISA, endothelin 3 Kit ELISA, edn3.L Kit ELISA, EDN3 Kit ELISA, Edn3 Kit ELISA
    Sujet
    Alternative name: ET3, PPET3, Preproendothelin-3
    ID gène
    13616
    UniProt
    P48299
    Pathways
    Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones
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