Endothelin 3 Kit ELISA
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- Antigène Voir toutes Endothelin 3 (EDN3) Kits ELISA
- Endothelin 3 (EDN3)
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 31.25 pg/mL - 2000 pg/mL
- Seuil minimal de détection
- 31.25 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensibilité
- 14.9 pg/mL
- Ingrédients
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Matériel non inclus
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
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- Antigène Voir toutes Endothelin 3 (EDN3) Kits ELISA
- Endothelin 3 (EDN3)
- Abstract
- EDN3 Produits
- Synonymes
- et-3 Kit ELISA, EDN3 Kit ELISA, ET-3 Kit ELISA, ET3 Kit ELISA, HSCR4 Kit ELISA, PPET3 Kit ELISA, WS4B Kit ELISA, 114CH19 Kit ELISA, ls Kit ELISA, tmgc48 Kit ELISA, Et3 Kit ELISA, RGD1564825 Kit ELISA, preproET-3 Kit ELISA, endothelin-3 L homeolog Kit ELISA, endothelin 3 Kit ELISA, edn3.L Kit ELISA, EDN3 Kit ELISA, Edn3 Kit ELISA
- Sujet
- Alternative name: ET3, PPET3, Preproendothelin-3
- ID gène
- 13616
- UniProt
- P48299
- Pathways
- Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones
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