GRIN1/NMDAR1 Kit ELISA

N° du produit ABIN5674688

Détail du produit GRIN1/NMDAR1 Kit ELISA

Antigène: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0122 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Autre désignation: Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) (GRIN1 Produits)

Synonymes: GluN1 Kit ELISA, MRD8 Kit ELISA, NMDA1 Kit ELISA, NMDAR1 Kit ELISA, NR1 Kit ELISA, nmda1 Kit ELISA, nmdar1 Kit ELISA, GB19253 Kit ELISA, GRIN1 Kit ELISA, NMD-R1 Kit ELISA, Z16 Kit ELISA, GluRdelta1 Kit ELISA, GluRzeta1 Kit ELISA, M100174 Kit ELISA, Nmdar Kit ELISA, Rgsc174 Kit ELISA, NMDAR1.1 Kit ELISA, zgc:152678 Kit ELISA, glutamate ionotropic receptor NMDA type subunit 1 Kit ELISA, glutamate receptor, ionotropic, N-methyl D-aspartate 1 S homeolog Kit ELISA, NMDA receptor 1 Kit ELISA, G protein-regulated inducer of neurite outgrowth 1 Kit ELISA, glutamate receptor, ionotropic, NMDA1 (zeta 1) Kit ELISA, glutamate receptor, ionotropic, N-methyl D-aspartate 1a Kit ELISA, GRIN1 Kit ELISA, grin1.S Kit ELISA, grin1 Kit ELISA, Grin1 Kit ELISA, Nmdar1 Kit ELISA, Gprin1 Kit ELISA, grin1a Kit ELISA

Sujet: Alternative name: NMDA1, NMDAR1, NR1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1

ID gène: 14810

UniProt: P35438

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity