HYAL1 Kit ELISA
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- Antigène Voir toutes HYAL1 Kits ELISA
- HYAL1 (Hyaluronidase-1 (HYAL1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.312 ng/mL - 20 ng/mL
- Seuil minimal de détection
- 0.312 ng/mL
- Application
- ELISA
- Type d'échantillon
- Tissue Homogenate
- Analytical Method
- Quantitative
- Sensibilité
- 0129 ng/mL
- Ingrédients
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Matériel non inclus
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Hyaluronidase-1 (HYAL1) ELISA Kit
HYAL1 Reactivité: Humain Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Hyaluronidase-1 (HYAL1) ELISA KitHYAL1 Reactivité: Humain Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Hyaluronidase-1 (HYAL1) ELISA KitHYAL1 Reactivité: Rat Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
Hyaluronidase-1 (HYAL1) ELISA KitHYAL1 Reactivité: Boeuf (Vache) Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Hyaluronidase-1 (HYAL1) ELISA KitHYAL1 Reactivité: Humain AA 22-435 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA - heparin), Serum
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
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- Antigène Voir toutes HYAL1 Kits ELISA
- HYAL1 (Hyaluronidase-1 (HYAL1))
- Autre désignation
- Hyaluronoglucosaminidase 1 (HYAL1) (HYAL1 Produits)
- Synonymes
- HYAL-1 Kit ELISA, LUCA1 Kit ELISA, NAT6 Kit ELISA, Hya1 Kit ELISA, Hyal-1 Kit ELISA, hyaluronoglucosaminidase 1 Kit ELISA, HYAL1 Kit ELISA, Hyal1 Kit ELISA
- Sujet
- Alternative name: LUCA1, NAT6, Hyaluronidase-1, Lung carcinoma protein 1
- ID gène
- 3373
- UniProt
- Q12794
- Pathways
- Glycosaminoglycan Metabolic Process
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