Nodal Kit ELISA

N° du produit ABIN5674705

Détail du produit Nodal Kit ELISA

Antigène: Nodal (NODAL) (Nodal Homolog (NODAL))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.625 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.625 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 6.0 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: Nodal (NODAL) (Nodal Homolog (NODAL))

Abstract: NODAL Produits

Synonymes: NODAL Kit ELISA, Tg.413d Kit ELISA, HTX5 Kit ELISA, nodal4 Kit ELISA, nodal4-A Kit ELISA, nr4 Kit ELISA, xnr4 Kit ELISA, Xnr-1 Kit ELISA, nodal Kit ELISA, nr1 Kit ELISA, nr1-A Kit ELISA, nodal growth differentiation factor Kit ELISA, nodal homolog (mouse) Kit ELISA, nodal Kit ELISA, nodal growth differentiation factor L homeolog Kit ELISA, nodal homolog 1 L homeolog Kit ELISA, Nodal Kit ELISA, NODAL Kit ELISA, nodal.L Kit ELISA, nodal1.L Kit ELISA

ID gène: 4838

UniProt: Q96S42

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of Endopeptidase Activity