Neurocan Kit ELISA
NCAN
Reactivité: Souris
Colorimetric
Sandwich ELISA
0.781 ng/mL - 50 ng/mL
Tissue Homogenate
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- Antigène Voir toutes Neurocan (NCAN) Kits ELISA
- Neurocan (NCAN)
-
Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.781 ng/mL - 50 ng/mL
- Seuil minimal de détection
- 0.781 ng/mL
- Application
- ELISA
- Type d'échantillon
- Tissue Homogenate
- Analytical Method
- Quantitative
- Sensibilité
- 0.3 ng/mL
- Ingrédients
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Matériel non inclus
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product NCAN Kit ELISA
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Neurocan (NCAN) ELISA Kit
NCAN Reactivité: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
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- Antigène Voir toutes Neurocan (NCAN) Kits ELISA
- Neurocan (NCAN)
- Abstract
- NCAN Produits
- Synonymes
- cspg3 Kit ELISA, ncan Kit ELISA, si:ch211-123b7.1 Kit ELISA, NCAN Kit ELISA, CSPG3 Kit ELISA, Cspg3 Kit ELISA, C230035B04 Kit ELISA, Cspg3-rs Kit ELISA, Tgfbit Kit ELISA, neurocan b Kit ELISA, neurocan Kit ELISA, ncanb Kit ELISA, NCAN Kit ELISA, Ncan Kit ELISA
- Sujet
- Alternative name: CSPG3, NEUR, Neurocan Core Protein, Chondroitin Sulfate Proteoglycan 3, Neurocan Proteoglycan
- ID gène
- 13004
- UniProt
- P55066
- Pathways
- Glycosaminoglycan Metabolic Process
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