OPTN Kit ELISA

N° du produit ABIN5674717

Détail du produit OPTN Kit ELISA

Antigène: OPTN (Optineurin (OPTN))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 34.56 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: OPTN (Optineurin (OPTN))

Abstract: OPTN Produits

Synonymes: nrp Kit ELISA, fip2 Kit ELISA, hip7 Kit ELISA, hypl Kit ELISA, glc1e Kit ELISA, tfiiia-intp Kit ELISA, ALS12 Kit ELISA, FIP2 Kit ELISA, GLC1E Kit ELISA, HIP7 Kit ELISA, HYPL Kit ELISA, NRP Kit ELISA, TFIIIA-INTP Kit ELISA, Fip2 Kit ELISA, 4930441O07Rik Kit ELISA, fj52f04 Kit ELISA, si:ch211-240l19.3 Kit ELISA, wu:fj52f04 Kit ELISA, zgc:66386 Kit ELISA, zgc:77868 Kit ELISA, optineurin Kit ELISA, optineurin L homeolog Kit ELISA, OPTN Kit ELISA, optn Kit ELISA, Optn Kit ELISA, optn.L Kit ELISA

Sujet: Alternative name: NRP, FIP2, GLC1E, HIP7, HYPL, TFIIIA-INTP, Glaucoma 1,Open Angle,E(Adult-Onset), Huntingtin-interacting protein 7, Optic neuropathy-inducing protein, E3-14.7K-interacting protein

ID gène: 10133

UniProt: Q96CV9

Pathways: M Phase