PIM1 Kit ELISA

N° du produit ABIN5674719

Détail du produit PIM1 Kit ELISA

Antigène: PIM1 (Pim-1 Oncogene (PIM1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0068 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: PIM1 (Pim-1 Oncogene (PIM1))

Abstract: PIM1 Produits

Synonymes: PIM Kit ELISA, Pim-1 Kit ELISA, PIM1 Kit ELISA, pim Kit ELISA, pim-1 Kit ELISA, pim3 Kit ELISA, Pim-1 proto-oncogene, serine/threonine kinase Kit ELISA, proviral integration site 1 Kit ELISA, Pim-1 proto-oncogene, serine/threonine kinase L homeolog Kit ELISA, PIM1 Kit ELISA, Pim1 Kit ELISA, pim1 Kit ELISA, pim1.L Kit ELISA

Sujet: Alternative name: Proto-Oncogene Serine/Threonine-Protein Kinase Pim-1

ID gène: 24649

UniProt: P26794

Pathways: Glycosaminoglycan Metabolic Process