MAT1A Kit ELISA

N° du produit ABIN5674721

Détail du produit MAT1A Kit ELISA

Antigène: MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.625 ng/mL - 40 ng/mL

Seuil minimal de détection: 0.625 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0255 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: MAT1A (Methionine Adenosyltransferase I, alpha (MAT1A))

Autre désignation: Methionine Adenosyltransferase I Alpha (MAT1a) (MAT1A Produits)

Synonymes: MAT Kit ELISA, MATA1 Kit ELISA, SAMS Kit ELISA, SAMS1 Kit ELISA, AdoMet Kit ELISA, SADE Kit ELISA, SAS Kit ELISA, AI046368 Kit ELISA, Ams Kit ELISA, wu:fi35e01 Kit ELISA, zgc:55442 Kit ELISA, methionine adenosyltransferase 1A Kit ELISA, methionine adenosyltransferase I, alpha Kit ELISA, MAT1A Kit ELISA, Mat1a Kit ELISA, mat1a Kit ELISA

Sujet: Alternative name: MAT, SAMS, MATA1, SAMS1, AMS1, S-Adenosylmethionine Synthetase, S-adenosylmethionine synthase isoform type-1

ID gène: 4143

UniProt: Q00266

Pathways: Mitotic G1-G1/S Phases, M Phase, Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process