SREBF1 Kit ELISA

N° du produit ABIN5674726

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat SREBF1.

Aperçu rapide pour SREBF1 Kit ELISA (ABIN5674726)

Antigène: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.39 ng/mL - 25 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Détail du produit SREBF1 Kit ELISA

Seuil minimal de détection: 0.39 ng/mL

Analytical Method: Quantitative

Sensibilité: 0144 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Sujet: Alternative name: SREBP1, Sterol Regulatory Element-Binding Protein 1, Adipocyte determination- and differentiation-dependent factor 1, ADD1

ID gène: 78968

UniProt: P56720

Pathways: AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha