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SYN1 Kit ELISA

SYN1 Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
N° du produit ABIN5674729
  • Antigène Voir toutes SYN1 Kits ELISA
    SYN1 (Synapsin I (SYN1))
    Reactivité
    • 4
    • 4
    • 4
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0148 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes SYN1 Kits ELISA
    SYN1 (Synapsin I (SYN1))
    Abstract
    SYN1 Produits
    Synonymes
    SYN1a Kit ELISA, SYN1b Kit ELISA, SYNI Kit ELISA, Syn-1 Kit ELISA, SYN I Kit ELISA, si:dkey-90n12.3 Kit ELISA, synapsin I Kit ELISA, synapsin I L homeolog Kit ELISA, SYN1 Kit ELISA, Syn1 Kit ELISA, syn1.L Kit ELISA, syn1 Kit ELISA
    Sujet
    Alternative name: SYN1a, SYN1b, SYNI, Brain protein 4.1
    ID gène
    6853
    UniProt
    P17600
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