TUBB1 Kit ELISA
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- Antigène Voir toutes TUBB1 Kits ELISA
- TUBB1 (Tubulin, beta 1 (TUBB1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 78.125 pg/mL - 5000 pg/mL
- Seuil minimal de détection
- 78.125 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Sensibilité
- 30.8 pg/mL
- Ingrédients
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Matériel non inclus
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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Tubulin, beta 1 (TUBB1) ELISA Kit
TUBB1 Reactivité: Humain Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tubulin, beta 1 (TUBB1) ELISA KitTUBB1 Reactivité: Souris Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
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- Antigène Voir toutes TUBB1 Kits ELISA
- TUBB1 (Tubulin, beta 1 (TUBB1))
- Autre désignation
- Tubulin Beta 1 (TUBb1) (TUBB1 Produits)
- Synonymes
- TUBB3 Kit ELISA, 2810484G07Rik Kit ELISA, M(beta)1 Kit ELISA, TUBB1 Kit ELISA, tubulin beta 1 class VI Kit ELISA, tubulin, beta 1 class VI Kit ELISA, TUBB1 Kit ELISA, Tubb1 Kit ELISA
- ID gène
- 81027
- UniProt
- Q9H4B7
- Pathways
- Dynamique des Microtubules
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