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VLDLR Kit ELISA

VLDLR Reactivité: Souris Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
N° du produit ABIN5674735
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    VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))
    Reactivité
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    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.56 ng/mL - 100 ng/mL
    Seuil minimal de détection
    1.56 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0.53 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes VLDLR Kits ELISA
    VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))
    Abstract
    VLDLR Produits
    Synonymes
    CARMQ1 Kit ELISA, CHRMQ1 Kit ELISA, VLDLRCH Kit ELISA, AA408956 Kit ELISA, AI451093 Kit ELISA, AW047288 Kit ELISA, vldlr Kit ELISA, very low density lipoprotein receptor Kit ELISA, very low density lipoprotein receptor S homeolog Kit ELISA, VLDLR Kit ELISA, Vldlr Kit ELISA, vldlr.S Kit ELISA
    Sujet
    Alternative name: VLDL-R
    ID gène
    22359
    UniProt
    P98156
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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