ZP2 Kit ELISA

N° du produit ABIN5674738

Détail du produit ZP2 Kit ELISA

Antigène: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0058 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Autre désignation: Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) (ZP2 Produits)

Synonymes: fa12e07 Kit ELISA, wu:fa12e07 Kit ELISA, zgc:152728 Kit ELISA, zp2 Kit ELISA, Zp-2 Kit ELISA, ZPA Kit ELISA, zona pellucida glycoprotein 2, tandem duplicate 5 Kit ELISA, zona pellucida glycoprotein 2 Kit ELISA, zp2.5 Kit ELISA, Zp2 Kit ELISA, ZP2 Kit ELISA

Sujet: Alternative name: ZPA, Zona Pellucida Sperm-Binding Protein 1 , Zona pellucida protein A

ID gène: 7783

UniProt: Q05996