GBA Kit ELISA

N° du produit ABIN5674742

Détail du produit GBA Kit ELISA

Antigène: GBA (Glucosidase, Beta, Acid (GBA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0064 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: GBA (Glucosidase, Beta, Acid (GBA))

Autre désignation: Glucosidase Beta, Acid (GbA) (GBA Produits)

Synonymes: GBA1 Kit ELISA, GCB Kit ELISA, GLUC Kit ELISA, BETA-GLUCOSIDASE Kit ELISA, T12J13.8 Kit ELISA, T12J13_8 Kit ELISA, beta glucosidase 25 Kit ELISA, PSPTO3318 Kit ELISA, PSPTO4290 Kit ELISA, GC Kit ELISA, GCase Kit ELISA, betaGC Kit ELISA, glucosylceramidase beta Kit ELISA, beta glucosidase 25 Kit ELISA, beta-glucosidase Kit ELISA, Beta-glucosidase Kit ELISA, glucosidase, beta, acid Kit ELISA, glucosylceramidase Kit ELISA, bglX-2 Kit ELISA, GBA Kit ELISA, BGLU25 Kit ELISA, PSPTO_3318 Kit ELISA, bglX Kit ELISA, bglA4 Kit ELISA, Gba Kit ELISA, LOC100399524 Kit ELISA, bglA.2 Kit ELISA, bglX-2 Kit ELISA

Sujet: Alternative name: GBA1, GCB, GLUC, Imiglucerase, Alglucerase, Beta-glucocerebrosidase, Glucosylceramidase, β-Glucosidase, D-Glucosyl-N-Acylsphingosine Glucohydrolase

ID gène: 14466

UniProt: P17439

Pathways: Cellular Glucan Metabolic Process