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LCT Kit ELISA

LCT Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
N° du produit ABIN5674743
  • Antigène Voir toutes LCT Kits ELISA
    LCT (Lactase (LCT))
    Reactivité
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0.05 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes LCT Kits ELISA
    LCT (Lactase (LCT))
    Abstract
    LCT Produits
    Synonymes
    LAC Kit ELISA, LPH Kit ELISA, LPH1 Kit ELISA, Lph Kit ELISA, Gm100 Kit ELISA, Lphl Kit ELISA, LCT Kit ELISA, PNGH Kit ELISA, GLH1 Kit ELISA, lac Kit ELISA, lct Kit ELISA, lph Kit ELISA, lph1 Kit ELISA, lactase Kit ELISA, lactase Kit ELISA, lactase, gene 1 L homeolog Kit ELISA, lactase, gene 2 L homeolog Kit ELISA, lactase, gene 2 Kit ELISA, lactase phlorizin hydrolase Kit ELISA, LCT Kit ELISA, Lct Kit ELISA, lct.1.L Kit ELISA, lct.2.L Kit ELISA, lct.2 Kit ELISA, LPH Kit ELISA
    Sujet
    Alternative name: LAC, LPH, LPH1, Lactose-Phlorizin Hydrolase
    ID gène
    3938
    UniProt
    P09848
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