LCT Kit ELISA
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- Antigène Voir toutes LCT Kits ELISA
- LCT (Lactase (LCT))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Tissue Homogenate
- Analytical Method
- Quantitative
- Sensibilité
- 0.05 ng/mL
- Ingrédients
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Matériel non inclus
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
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- Antigène Voir toutes LCT Kits ELISA
- LCT (Lactase (LCT))
- Abstract
- LCT Produits
- Synonymes
- LAC Kit ELISA, LPH Kit ELISA, LPH1 Kit ELISA, Lph Kit ELISA, Gm100 Kit ELISA, Lphl Kit ELISA, LCT Kit ELISA, PNGH Kit ELISA, GLH1 Kit ELISA, lac Kit ELISA, lct Kit ELISA, lph Kit ELISA, lph1 Kit ELISA, lactase Kit ELISA, lactase Kit ELISA, lactase, gene 1 L homeolog Kit ELISA, lactase, gene 2 L homeolog Kit ELISA, lactase, gene 2 Kit ELISA, lactase phlorizin hydrolase Kit ELISA, LCT Kit ELISA, Lct Kit ELISA, lct.1.L Kit ELISA, lct.2.L Kit ELISA, lct.2 Kit ELISA, LPH Kit ELISA
- Sujet
- Alternative name: LAC, LPH, LPH1, Lactose-Phlorizin Hydrolase
- ID gène
- 3938
- UniProt
- P09848
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