SESN3 Kit ELISA

N° du produit ABIN5674746

Détail du produit SESN3 Kit ELISA

Antigène: SESN3 (Sestrin 3 (SESN3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0056 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: SESN3 (Sestrin 3 (SESN3))

Abstract: SESN3 Produits

Synonymes: cb261 Kit ELISA, sb:cb261 Kit ELISA, zgc:56722 Kit ELISA, wu:fb77c04 Kit ELISA, wu:fc41d03 Kit ELISA, wu:fd59d12 Kit ELISA, sestrin-3 Kit ELISA, sest3 Kit ELISA, SEST3 Kit ELISA, 5630400E15Rik Kit ELISA, BC003348 Kit ELISA, sestrin 3 Kit ELISA, sestrin 3 L homeolog Kit ELISA, SESN3 Kit ELISA, sesn3 Kit ELISA, sesn3.L Kit ELISA, Sesn3 Kit ELISA

Sujet: Alternative name: SEST3

ID gène: 143686

UniProt: P58005