CCR2 Kit ELISA

N° du produit ABIN5674764

Détail du produit CCR2 Kit ELISA

Antigène: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0061 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Autre désignation: Chemokine C-C-Motif Receptor 2 (CCR2) (CCR2 Produits)

Synonymes: CC-CKR-2 Kit ELISA, CCR-2 Kit ELISA, CCR2A Kit ELISA, CCR2B Kit ELISA, CD192 Kit ELISA, CKR2 Kit ELISA, CKR2A Kit ELISA, CKR2B Kit ELISA, CMKBR2 Kit ELISA, MCP-1-R Kit ELISA, ACKR5 Kit ELISA, CKRX Kit ELISA, CRAM Kit ELISA, CRAM-A Kit ELISA, CRAM-B Kit ELISA, HCR Kit ELISA, Cc-ckr-2 Kit ELISA, Ccr2a Kit ELISA, Ccr2b Kit ELISA, Ckr2 Kit ELISA, Ckr2a Kit ELISA, Ckr2b Kit ELISA, Cmkbr2 Kit ELISA, mJe-r Kit ELISA, C-C motif chemokine receptor 2 Kit ELISA, C-C motif chemokine receptor like 2 Kit ELISA, chemokine (C-C motif) receptor 2 Kit ELISA, CCR2 Kit ELISA, CCRL2 Kit ELISA, Ccr2 Kit ELISA, LOC484790 Kit ELISA

Sujet: Alternative name: CD192, CC-CKR2, CCR2A, C-C CKR-2, CCR2B, CKR2-A, CKR2-B, CMKBR2, MCP1-R, Monocyte chemoattractant protein 1 receptor

ID gène: 12772

UniProt: P51683

Pathways: cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process