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NEU2 Kit ELISA

NEU2 Reactivité: Souris Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5674768
  • Antigène Voir toutes NEU2 Kits ELISA
    NEU2 (Sialidase 2 (Cytosolic Sialidase) (NEU2))
    Reactivité
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0085 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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    Discover our top product NEU2 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes NEU2 Kits ELISA
    NEU2 (Sialidase 2 (Cytosolic Sialidase) (NEU2))
    Autre désignation
    Sialidase 2, Cytosolic (SIAL2) (NEU2 Produits)
    Synonymes
    NEU2 Kit ELISA, MBS Kit ELISA, MSS Kit ELISA, MTS Kit ELISA, SIAL2 Kit ELISA, neuraminidase 2 Kit ELISA, sialidase 2 (cytosolic sialidase) Kit ELISA, NEU2 Kit ELISA, Neu2 Kit ELISA
    Sujet
    Alternative name: NEU2, N-Acetyl-Alpha-Neuraminidase 2, Cytosolic sialidase
    ID gène
    23956
    UniProt
    Q9JMH3
    Pathways
    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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