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AVPR1B Kit ELISA

AVPR1B Reactivité: Rat Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Tissue Homogenate
N° du produit ABIN5674780
  • Antigène Voir toutes AVPR1B Kits ELISA
    AVPR1B (Arginine Vasopressin Receptor 1B (AVPR1B))
    Reactivité
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.625 ng/mL - 40 ng/mL
    Seuil minimal de détection
    0.625 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0266 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes AVPR1B Kits ELISA
    AVPR1B (Arginine Vasopressin Receptor 1B (AVPR1B))
    Abstract
    AVPR1B Produits
    Synonymes
    LOC443050 Kit ELISA, AVPR1B Kit ELISA, si:ch211-125a15.3 Kit ELISA, AVPR3 Kit ELISA, V3/V1b Kit ELISA, VIBR Kit ELISA, VPR3 Kit ELISA, arginine vasopressin receptor 1B Kit ELISA, arginine vasopressin receptor 1Ab Kit ELISA, AVPR1B Kit ELISA, avpr1ab Kit ELISA, Avpr1b Kit ELISA
    Sujet
    Alternative name: AVPR1-B, AVPR3, Vasopressin 3 Receptor, Antidiuretic Hormone Receptor 1b
    ID gène
    29462
    UniProt
    P48974
    Pathways
    Regulation of Systemic Arterial Blood Pressure by Hormones
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