HAS2 Kit ELISA

N° du produit ABIN5674787

Détail du produit HAS2 Kit ELISA

Antigène: HAS2 (Hyaluronan Synthase 2 (HAS2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 26 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: HAS2 (Hyaluronan Synthase 2 (HAS2))

Abstract: HAS2 Produits

Synonymes: DG42 Kit ELISA, XHas2 Kit ELISA, Has2 Kit ELISA, hyaluronan synthase 2 Kit ELISA, hyaluronan synthase 2 S homeolog Kit ELISA, has2 Kit ELISA, HAS2 Kit ELISA, Has2 Kit ELISA, SHAS2 Kit ELISA, has2.S Kit ELISA

Sujet: Alternative name: Hyaluronate synthase 2, Hyaluronic acid synthase 2

ID gène: 15117

UniProt: P70312

Pathways: Glycosaminoglycan Metabolic Process