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Utrophin Kit ELISA

UTRN Reactivité: Humain Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5674792
  • Antigène Voir toutes Utrophin (UTRN) Kits ELISA
    Utrophin (UTRN)
    Reactivité
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.625 ng/mL - 40 ng/mL
    Seuil minimal de détection
    0.625 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0252 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes Utrophin (UTRN) Kits ELISA
    Utrophin (UTRN)
    Abstract
    UTRN Produits
    Synonymes
    drp Kit ELISA, dmdl Kit ELISA, drp1 Kit ELISA, DKFZp469A0710 Kit ELISA, DMDL Kit ELISA, DRP Kit ELISA, DRP1 Kit ELISA, AA589569 Kit ELISA, Dmdl Kit ELISA, utrophin L homeolog Kit ELISA, utrophin Kit ELISA, utrn.L Kit ELISA, UTRN Kit ELISA, LOAG_03796 Kit ELISA, utrn Kit ELISA, LOC100551145 Kit ELISA, Utrn Kit ELISA
    Sujet
    Alternative name: DRP1, DMDL, DRP, Homologous To Dystrophin, Dystrophin-related protein 1
    ID gène
    7402
    UniProt
    P46939
    Pathways
    Skeletal Muscle Fiber Development
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