CD73 Kit ELISA

N° du produit ABIN5674798

Détail du produit CD73 Kit ELISA

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 26.1 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Autre désignation: 5'-Nucleotidase, Ecto (NT5E) (NT5E Produits)

Synonymes: CALJA Kit ELISA, CD73 Kit ELISA, E5NT Kit ELISA, NT Kit ELISA, NT5 Kit ELISA, NTE Kit ELISA, eN Kit ELISA, eNT Kit ELISA, zgc:63784 Kit ELISA, NT5E Kit ELISA, MGC114869 Kit ELISA, PSPTO2277 Kit ELISA, 2210401F01Rik Kit ELISA, AI447961 Kit ELISA, Nt5 Kit ELISA, 5'-nucleotidase ecto Kit ELISA, 5'-nucleotidase, ecto (CD73) Kit ELISA, 5'-nucleotidase Kit ELISA, 5' nucleotidase, ecto Kit ELISA, NT5E Kit ELISA, nt5e Kit ELISA, nt5e.S Kit ELISA, PSPTO_2277 Kit ELISA, CBU_1233 Kit ELISA, MCA1322 Kit ELISA, Nt5e Kit ELISA

Sujet: Alternative name: CD73, NT5-E, E5NT, E5-NT, NTE, EN, ENT

ID gène: 23959

UniProt: Q61503

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process