ALDOA Kit ELISA

N° du produit ABIN5674804

Détail du produit ALDOA Kit ELISA

Antigène: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 34.5 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ALDOA (Aldolase A, Fructose-Bisphosphate (ALDOA))

Autre désignation: Aldolase A, Fructose Bisphosphate (ALDOA) (ALDOA Produits)

Synonymes: ALDA Kit ELISA, GSD12 Kit ELISA, aldoa Kit ELISA, cb79 Kit ELISA, sb:cb79 Kit ELISA, wu:fa28b10 Kit ELISA, wu:fb10b11 Kit ELISA, ALDOA Kit ELISA, Aldo-1 Kit ELISA, Aldo1 Kit ELISA, RNALDOG5 Kit ELISA, hm:zeh0036 Kit ELISA, zgc:77696 Kit ELISA, aldolase, fructose-bisphosphate A Kit ELISA, aldolase a, fructose-bisphosphate, a Kit ELISA, aldolase, fructose-bisphosphate A S homeolog Kit ELISA, aldolase A, fructose-bisphosphate Kit ELISA, aldolase a, fructose-bisphosphate, b Kit ELISA, ALDOA Kit ELISA, aldoaa Kit ELISA, aldoa Kit ELISA, aldoa.S Kit ELISA, Aldoa Kit ELISA, aldoab Kit ELISA

Sujet: Alternative name: FBA, ALDO-A, Fructose 1,6 Bisphosphate Aldolase, Lung cancer antigen NY-LU-1, Muscle-type aldolase

ID gène: 11674

UniProt: P05064

Pathways: Ribonucleoside Biosynthetic Process