UGCG Kit ELISA

N° du produit ABIN5674821

Détail du produit UGCG Kit ELISA

Antigène: UGCG (UDP-Glucose Ceramide Glucosyltransferase (UGCG))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.625 ng/mL - 40 ng/mL

Seuil minimal de détection: 0.625 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0216 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: UGCG (UDP-Glucose Ceramide Glucosyltransferase (UGCG))

Autre désignation: UDP Glucose Ceramide Glucosyltransferase (UGCG) (UGCG Produits)

Synonymes: GCS Kit ELISA, GLCT1 Kit ELISA, XLCGT Kit ELISA, gcs Kit ELISA, glct1 Kit ELISA, ugcga Kit ELISA, xlcgt Kit ELISA, cb539 Kit ELISA, sb:cb539 Kit ELISA, zgc:112506 Kit ELISA, ugcg Kit ELISA, ugcgb Kit ELISA, AU043821 Kit ELISA, C80537 Kit ELISA, Epcs21 Kit ELISA, GlcT-1 Kit ELISA, Ugcgl Kit ELISA, UDP-glucose ceramide glucosyltransferase Kit ELISA, UDP-glucose ceramide glucosyltransferase L homeolog Kit ELISA, UDP-glucose ceramide glucosyltransferase S homeolog Kit ELISA, UGCG Kit ELISA, ugcg.L Kit ELISA, Ugcg Kit ELISA, ugcg Kit ELISA, ugcg.S Kit ELISA

Sujet: Alternative name: GCS, GLCT-1, Glucosylceramide Synthase, N-acylsphingosine D-glucosyltransferase

ID gène: 83626

UniProt: Q9R0E0