DAB1 Kit ELISA

N° du produit ABIN5674825

Détail du produit DAB1 Kit ELISA

Antigène: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0139 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Autre désignation: Disabled Homolog 1 (DAB1) (DAB1 Produits)

Synonymes: AI956902 Kit ELISA, C630028C02Rik Kit ELISA, scm Kit ELISA, scr Kit ELISA, scrambler Kit ELISA, yot Kit ELISA, disabled-1 Kit ELISA, LOC100232823 Kit ELISA, si:dkey-242m13.1 Kit ELISA, DAB1 Kit ELISA, zgc:136547 Kit ELISA, DAB1, reelin adaptor protein Kit ELISA, disabled 1 Kit ELISA, disabled homolog 1 Kit ELISA, Dab, reelin signal transducer, homolog 1a (Drosophila) Kit ELISA, Dab, reelin signal transducer, homolog 1b (Drosophila) Kit ELISA, DAB1 Kit ELISA, Dab1 Kit ELISA, LOC100232823 Kit ELISA, dab1a Kit ELISA, dab1b Kit ELISA

ID gène: 1600

UniProt: O75553