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ACAT1 Kit ELISA

ACAT1 Reactivité: Rat Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5674861
  • Antigène Voir toutes ACAT1 Kits ELISA
    ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))
    Reactivité
    • 2
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.25 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.25 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    12.5 pg/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes ACAT1 Kits ELISA
    ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))
    Autre désignation
    Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) (ACAT1 Produits)
    Synonymes
    ACAT Kit ELISA, MAT Kit ELISA, T2 Kit ELISA, THIL Kit ELISA, RATACAL Kit ELISA, 6330585C21Rik Kit ELISA, Acat Kit ELISA, fd16h07 Kit ELISA, fd20g06 Kit ELISA, wu:fd16h07 Kit ELISA, wu:fd20g06 Kit ELISA, zgc:86832 Kit ELISA, acat1-a Kit ELISA, acetyl-CoA acetyltransferase 1 Kit ELISA, acetyl-Coenzyme A acetyltransferase 1 Kit ELISA, acetyl-CoA acetyltransferase 1 L homeolog Kit ELISA, ACAT1 Kit ELISA, Acat1 Kit ELISA, acat1 Kit ELISA, acat1.L Kit ELISA
    Sujet
    Alternative name: THIL, ACAT, T2, MAT, Acetoacetyl Coenzyme A Thiolase
    ID gène
    25014
    UniProt
    P17764
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