CPB1 Kit ELISA
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- Antigène Voir toutes CPB1 Kits ELISA
- CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensibilité
- 0055 ng/mL
- Ingrédients
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Matériel non inclus
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Durée du test
- 1 - 4.5 h
- Plaque
- Pre-coated
- Protocole
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Stock
- 4 °C
- Date de péremption
- 12 months
-
- Antigène Voir toutes CPB1 Kits ELISA
- CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))
- Autre désignation
- Carboxypeptidase B1, Tissue (CPB1) (CPB1 Produits)
- Synonymes
- wu:fb60d07 Kit ELISA, wu:fb69b08 Kit ELISA, CPB1 Kit ELISA, pCPB Kit ELISA, CPB Kit ELISA, PASP Kit ELISA, PCPB Kit ELISA, Cpb Kit ELISA, 0910001A18Rik Kit ELISA, 1810063F02Rik Kit ELISA, 2210008M23Rik Kit ELISA, AI504870 Kit ELISA, carboxypeptidase B1 (tissue) Kit ELISA, carboxypeptidase B1 Kit ELISA, carboxypeptidase B1 S homeolog Kit ELISA, carboxypeptidase B Kit ELISA, cpb1 Kit ELISA, CPB1 Kit ELISA, cpb1.S Kit ELISA, CpipJ_CPIJ010799 Kit ELISA, CpipJ_CPIJ010801 Kit ELISA, VDBG_02663 Kit ELISA, LOC100562609 Kit ELISA, Cpb1 Kit ELISA
- Sujet
- Alternative name: CPB, PCPB, PASP, Pancreas-specific protein
- ID gène
- 1360
- UniProt
- P15086
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