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CPB1 Kit ELISA

CPB1 Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
N° du produit ABIN5674875
  • Antigène Voir toutes CPB1 Kits ELISA
    CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))
    Reactivité
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Sensibilité
    0055 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes CPB1 Kits ELISA
    CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))
    Autre désignation
    Carboxypeptidase B1, Tissue (CPB1) (CPB1 Produits)
    Synonymes
    wu:fb60d07 Kit ELISA, wu:fb69b08 Kit ELISA, CPB1 Kit ELISA, pCPB Kit ELISA, CPB Kit ELISA, PASP Kit ELISA, PCPB Kit ELISA, Cpb Kit ELISA, 0910001A18Rik Kit ELISA, 1810063F02Rik Kit ELISA, 2210008M23Rik Kit ELISA, AI504870 Kit ELISA, carboxypeptidase B1 (tissue) Kit ELISA, carboxypeptidase B1 Kit ELISA, carboxypeptidase B1 S homeolog Kit ELISA, carboxypeptidase B Kit ELISA, cpb1 Kit ELISA, CPB1 Kit ELISA, cpb1.S Kit ELISA, CpipJ_CPIJ010799 Kit ELISA, CpipJ_CPIJ010801 Kit ELISA, VDBG_02663 Kit ELISA, LOC100562609 Kit ELISA, Cpb1 Kit ELISA
    Sujet
    Alternative name: CPB, PCPB, PASP, Pancreas-specific protein
    ID gène
    1360
    UniProt
    P15086
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