GOT2 Kit ELISA

N° du produit ABIN5674876

Détail du produit GOT2 Kit ELISA

Antigène: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0089 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Autre désignation: Aspartate Aminotransferase 2 (AST2) (GOT2 Produits)

Synonymes: KAT4 Kit ELISA, KATIV Kit ELISA, mitAAT Kit ELISA, AI789014 Kit ELISA, Got-1 Kit ELISA, cAspAT Kit ELISA, cCAT Kit ELISA, AL022787 Kit ELISA, FABP-pm Kit ELISA, Got-2 Kit ELISA, mAspAT Kit ELISA, ASPATA Kit ELISA, got2 Kit ELISA, zgc:66329 Kit ELISA, fj40h07 Kit ELISA, wu:fj40h07 Kit ELISA, zgc:56425 Kit ELISA, glutamic-oxaloacetic transaminase 2 Kit ELISA, glutamic-oxaloacetic transaminase 1, soluble Kit ELISA, glutamatic-oxaloacetic transaminase 2, mitochondrial Kit ELISA, glutamic-oxaloacetic transaminase 2a, mitochondrial Kit ELISA, glutamic-oxaloacetic transaminase 2 L homeolog Kit ELISA, Aspartate amino transferase activity Kit ELISA, glutamic-oxaloacetic transaminase 2b, mitochondrial Kit ELISA, GOT2 Kit ELISA, got2 Kit ELISA, Got1 Kit ELISA, Got2 Kit ELISA, got2a Kit ELISA, got2.L Kit ELISA, AST Kit ELISA, got2b Kit ELISA

Sujet: Alternative name: GOT2, MitAAT, KATIV, KAT4, FABP-1, Plasma membrane-associated fatty acid-binding protein, Glutamic-Oxaloacetic Transaminase 2,Mitochondrial, Kynurenine Aminotransferase IV

ID gène: 2806

UniProt: P00505

Pathways: Monocarboxylic Acid Catabolic Process