ZEB2 Kit ELISA

N° du produit ABIN5674882

Détail du produit ZEB2 Kit ELISA

Antigène: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.25 ng/mL - 80 ng/mL

Seuil minimal de détection: 1.25 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0.6 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

Autre désignation: Zinc Finger Homeobox Protein 1B (ZFHX1B) (ZEB2 Produits)

Synonymes: ZFHX1B Kit ELISA, Zfhx1b Kit ELISA, HSPC082 Kit ELISA, SIP-1 Kit ELISA, SIP1 Kit ELISA, SMADIP1 Kit ELISA, 9130203F04Rik Kit ELISA, D130016B08Rik Kit ELISA, Zfx1b Kit ELISA, Zfxh1b Kit ELISA, mKIAA0569 Kit ELISA, XSip1 Kit ELISA, sip1 Kit ELISA, zfhx1b Kit ELISA, si:dkey-52o2.2 Kit ELISA, sip1a Kit ELISA, wu:fc92g08 Kit ELISA, sip1b Kit ELISA, zinc finger E-box binding homeobox 2 Kit ELISA, zinc finger E-box binding homeobox 2 S homeolog Kit ELISA, zinc finger homeobox protein Kit ELISA, zinc finger E-box binding homeobox 2a Kit ELISA, zinc finger E-box binding homeobox 2b Kit ELISA, ZEB2 Kit ELISA, Zeb2 Kit ELISA, zeb2.S Kit ELISA, zeb2 Kit ELISA, zeb2a Kit ELISA, zeb2b Kit ELISA

Sujet: Alternative name: ZEB2, SIP1, SIP-1, SMADIP1, SIP1, Zinc Finger E-Box Binding Homeobox 2, SMAD Interacting Protein 1

ID gène: 9839

UniProt: O60315

Pathways: Tube Formation